José Pissarra

Protein intracellular trafficking has been the main topic of research. Present collaboration with aerobiology group has tackled potential allergenic pollen proteins. Coding sequences of these proteins have been cloned and recombinant proteins produced to allow further allergenicity characterization. Changes in gene expression are evaluated by Western blotting and qPCR in pollen subjected to different atmospheric pollutants, confocal and immunoelectron microscopy is used for protein localization.