P. Baptista, E. Pereira, P. Eaton,
G. Doria, A. Miranda, I. Gomes,
P. Quaresma and R. Franco
Gold nanoparticles for the development of clinical
Analytical and Bioanalytical Chemistry, 391, 943-950 (2008).
The impact of advances in nanotechnology is particularly relevant in biodiagnostics, where nanoparticlebased assays have been developed for specific detection of bioanalytes of clinical interest. Gold nanoparticles showeasily
tuned physical properties, including unique optical properties, robustness, and high surface areas, making them ideal candidates for developing biomarker platforms. Modulation of these physicochemical properties can be easily achieved by adequate synthetic strategies and give gold nanoparticles advantages over conventional detection methods currently used in clinical diagnostics. The surface of gold nanoparticles can be tailored by ligand functionalization to selectively bind biomarkers. Thiol-linking of DNA and chemical functionalization
of gold nanoparticles for specific protein/antibody binding are the most common approaches. Simple and inexpensive methods based on these bio-nanoprobes were
initially applied for detection of specific DNA sequences and are presently being expanded to clinical diagnosis.
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